Sensitivity of Cancer cell line XYZ to Drug A

test

contact: mattpi@rs-email.net

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Date

2018-05-10

Last modified: Wed May 13 07:12:38 UTC 2026

Objective

Last modified: Wed May 13 07:12:38 UTC 2026

Method

Cells used from here: 2018-05-09 Plating of cell line XYZ Protocol - XTT cell proliferation assay <ol> <li>A dilution series of Drug A was made based on the calculations below.</li> <li>100 &micro;l of each dilution series was then plated in quadruplicate.</li> <li>Plates were incubated for 72 hrs and cell viability assessed using a XTT assay.</li> </ol> <table style="width: 559px;" border="1" cellspacing="0" cellpadding="6"><colgroup><col width="267" /> <col width="267" /> </colgroup> <tbody> <tr valign="TOP"> <td width="267"> Plate layout: &nbsp; <img id="164000-457" class="imageDropped inlineImageThumbnail" src="/image/getImage/164000-457/-3987600160506727222" alt="image" data-size="1000-1289" height="324" width="251" /> </td> <td width="267"> Calculations: &nbsp; Drug A Desired concentration 100 &micro;M Desired Volume: 4 ml &nbsp; Mix 0.057 ml of Stock Drug A + 3.943 ml of media to generate the top dilution. Next, serially dilute 1:10 (0.4 ml of previous dilution + 3.6 ml of media) Repeat from -1 dilution through to -11. &nbsp; 100 &micro;l of media is added to the bottom row of cells as the control. </td> </tr> </tbody> </table>

Last modified: Wed May 13 07:12:38 UTC 2026

Results

Following incubated for 72 hours, cells were observed under an inverted microscope. Cells in the top row (highest concentration of drug A were totally destroyed/non-viable). Cells in the media alone treatment wells were growing healthily. A XTT assay was performed (incubated for 4 h) and yielded the following results: <table border="1" cellspacing="0"><colgroup span="5" width="114"></colgroup> <tbody> <tr> <td align="LEFT" valign="BOTTOM" height="15">Log &micro;M</td> <td align="LEFT" valign="BOTTOM">Data in quadruplicate</td> <td align="LEFT" valign="BOTTOM">&nbsp;</td> <td align="LEFT" valign="BOTTOM">&nbsp;</td> <td align="LEFT" valign="BOTTOM">&nbsp;</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">2</td> <td align="RIGHT" valign="BOTTOM">0.602</td> <td align="RIGHT" valign="BOTTOM">0.594</td> <td align="RIGHT" valign="BOTTOM">0.611</td> <td align="RIGHT" valign="BOTTOM">0.602</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">1</td> <td align="RIGHT" valign="BOTTOM">0.578</td> <td align="RIGHT" valign="BOTTOM">0.544</td> <td align="RIGHT" valign="BOTTOM">0.515</td> <td align="RIGHT" valign="BOTTOM">0.52</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">0</td> <td align="RIGHT" valign="BOTTOM">0.668</td> <td align="RIGHT" valign="BOTTOM">0.662</td> <td align="RIGHT" valign="BOTTOM">0.633</td> <td align="RIGHT" valign="BOTTOM">0.666</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-1</td> <td align="RIGHT" valign="BOTTOM">0.829</td> <td align="RIGHT" valign="BOTTOM">0.847</td> <td align="RIGHT" valign="BOTTOM">0.749</td> <td align="RIGHT" valign="BOTTOM">0.795</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-2</td> <td align="RIGHT" valign="BOTTOM">0.731</td> <td align="RIGHT" valign="BOTTOM">0.773</td> <td align="RIGHT" valign="BOTTOM">0.801</td> <td align="RIGHT" valign="BOTTOM">0.777</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-3</td> <td align="RIGHT" valign="BOTTOM">1.345</td> <td align="RIGHT" valign="BOTTOM">1.156</td> <td align="RIGHT" valign="BOTTOM">1.115</td> <td align="RIGHT" valign="BOTTOM">1.238</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-4</td> <td align="RIGHT" valign="BOTTOM">1.869</td> <td align="RIGHT" valign="BOTTOM">0.763</td> <td align="RIGHT" valign="BOTTOM">2.119</td> <td align="RIGHT" valign="BOTTOM">2.33</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-5</td> <td align="RIGHT" valign="BOTTOM">1.984</td> <td align="RIGHT" valign="BOTTOM">1.948</td> <td align="RIGHT" valign="BOTTOM">2.023</td> <td align="RIGHT" valign="BOTTOM">2.266</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-6</td> <td align="RIGHT" valign="BOTTOM">1.916</td> <td align="RIGHT" valign="BOTTOM">1.879</td> <td align="RIGHT" valign="BOTTOM">1.964</td> <td align="RIGHT" valign="BOTTOM">2.317</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-7</td> <td align="RIGHT" valign="BOTTOM">2.03</td> <td align="RIGHT" valign="BOTTOM">1.938</td> <td align="RIGHT" valign="BOTTOM">2.169</td> <td align="RIGHT" valign="BOTTOM">2.451</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-8</td> <td align="RIGHT" valign="BOTTOM">1.968</td> <td align="RIGHT" valign="BOTTOM">1.837</td> <td align="RIGHT" valign="BOTTOM">1.916</td> <td align="RIGHT" valign="BOTTOM">2.203</td> </tr> <tr> <td align="LEFT" valign="BOTTOM" height="15">Media</td> <td align="RIGHT" valign="BOTTOM">2.114</td> <td align="RIGHT" valign="BOTTOM">2.179</td> <td align="RIGHT" valign="BOTTOM">2.114</td> <td align="RIGHT" valign="BOTTOM">2.179</td> </tr> </tbody> </table> Using Graphpad Prism to normalise the values, <table border="1" cellspacing="0"><colgroup span="5" width="114"></colgroup> <tbody> <tr> <td align="LEFT" valign="BOTTOM" height="15">Log &micro;M</td> <td align="LEFT" valign="BOTTOM">Normalized data</td> <td align="LEFT" valign="BOTTOM">&nbsp;</td> <td align="LEFT" valign="BOTTOM">&nbsp;</td> <td align="LEFT" valign="BOTTOM">&nbsp;</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">2</td> <td align="RIGHT" valign="BOTTOM">3.904183</td> <td align="RIGHT" valign="BOTTOM">3.4064372</td> <td align="RIGHT" valign="BOTTOM">4.4641457</td> <td align="RIGHT" valign="BOTTOM">3.904183</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">1</td> <td align="RIGHT" valign="BOTTOM">2.4109497</td> <td align="RIGHT" valign="BOTTOM">0.29553667</td> <td align="RIGHT" valign="BOTTOM">-1.5087901</td> <td align="RIGHT" valign="BOTTOM">-1.1977</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">0</td> <td align="RIGHT" valign="BOTTOM">8.010574</td> <td align="RIGHT" valign="BOTTOM">7.637267</td> <td align="RIGHT" valign="BOTTOM">5.832944</td> <td align="RIGHT" valign="BOTTOM">7.88614</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-1</td> <td align="RIGHT" valign="BOTTOM">18.027685</td> <td align="RIGHT" valign="BOTTOM">19.14761</td> <td align="RIGHT" valign="BOTTOM">13.050241</td> <td align="RIGHT" valign="BOTTOM">15.912272</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-2</td> <td align="RIGHT" valign="BOTTOM">11.930315</td> <td align="RIGHT" valign="BOTTOM">14.543473</td> <td align="RIGHT" valign="BOTTOM">16.28558</td> <td align="RIGHT" valign="BOTTOM">14.792346</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-3</td> <td align="RIGHT" valign="BOTTOM">50.132214</td> <td align="RIGHT" valign="BOTTOM">38.372993</td> <td align="RIGHT" valign="BOTTOM">35.822056</td> <td align="RIGHT" valign="BOTTOM">43.474876</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-4</td> <td align="RIGHT" valign="BOTTOM">82.734474</td> <td align="RIGHT" valign="BOTTOM">13.921293</td> <td align="RIGHT" valign="BOTTOM">98.288994</td> <td align="RIGHT" valign="BOTTOM">111.41701</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-5</td> <td align="RIGHT" valign="BOTTOM">89.88955</td> <td align="RIGHT" valign="BOTTOM">87.649704</td> <td align="RIGHT" valign="BOTTOM">92.31606</td> <td align="RIGHT" valign="BOTTOM">107.43506</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-6</td> <td align="RIGHT" valign="BOTTOM">85.65873</td> <td align="RIGHT" valign="BOTTOM">83.35665</td> <td align="RIGHT" valign="BOTTOM">88.64519</td> <td align="RIGHT" valign="BOTTOM">110.60817</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-7</td> <td align="RIGHT" valign="BOTTOM">92.75159</td> <td align="RIGHT" valign="BOTTOM">87.02753</td> <td align="RIGHT" valign="BOTTOM">101.39989</td> <td align="RIGHT" valign="BOTTOM">118.9454</td> </tr> <tr> <td align="RIGHT" valign="BOTTOM" height="15">-8</td> <td align="RIGHT" valign="BOTTOM">88.894066</td> <td align="RIGHT" valign="BOTTOM">80.7435</td> <td align="RIGHT" valign="BOTTOM">85.65873</td> <td align="RIGHT" valign="BOTTOM">103.51531</td> </tr> </tbody> </table><img id="164001-458" class="imageDropped inlineImageThumbnail" src="/image/getImage/164001-458/-3963803939001086230" alt="image" data-size="689-468" height="322" width="475" />The resulting Log Effective dose 50% was calculated to be -3.167 which is equivalent to an ED50 of 681.1 pM.

Last modified: Wed May 13 07:12:38 UTC 2026

Conclusion

Last modified: Wed May 13 07:12:38 UTC 2026

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Sensitivity of Cancer cell line XYZ to Drug A

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contact: mattpi@rs-email.net

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